To make the HiSeq RNA Sample Prep time-saving, Enrichment Kit, technical and biological replicates are available in the first kit run for more accurate and reliable quantification and visualization within the RNA QC Toolkit. TruSeq RNA Sample Prep time-saving, Enrichment Kit is designed for efficient capture and enrichment of all human transcripts. The DNA is amplified and indexed in a single-tube reaction for an average library size of 250 bases. In the setup of the kit, the capture process is followed by end repair, A-base addition (for the sequence ligation), sample purification, and gap repair.

Exome capture efficiency and coverage are optimized so that, on average, 99.4% of the exons are covered at greater than or equal to 17x and all 63 478 captured 21-mer genes are fully represented. The control library contains a PhiX spike-in and a set of in-house validated control DNAs to help with data analysis.

The TruSeq Exome Enrichment kit provides the following three components: the sequencing chemistry, a control library, and the gene-set containing 63 478 build 36 exons and flanking regions of 11044 genes. Significant improvements in the time for the capture and library preparation can be achieved by using this kit. The captured DNA can be applied on the HiSeq 2000 or HiSeq 2500 platform. The Illumina TruSeq Exome Enrichment Kit can be used to sequence paired-end libraries (in cluster format) or single-end libraries (in flow cells). This kit enables the targeted capture and enrichment of human exome in a streamlined workflow. can sequence exome capture targets in a paired-end format. The HiSeq Sample Preparation Kit by Illumina Co. Terraria All Items Map Download Ios !FULL!

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